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Rabbit SHANK3 Antibody
SKU: orb1523478
Description
Research Area
Neuroscience
Images & Validation
−Item 1 of 3
| Tested Applications | IP, WB |
|---|---|
| Dilution Range | WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Between 1475 and 1525 |
| Target | SHANK3 |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| Concentration | 1000 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−DEL22q13.3; proline rich synapse associated protein 2; Proline-rich synapse-associated protein 2; PROSAP2; PSAP2; SCZD15; SH3 and multiple ankyrin repeat domains protein 3; shank postsynaptic density protein; Shank3; SPANK-2
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Detection of mouse SHANK3 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SHANK3 antibody (orb1523478) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human SHANK3 by western blot. Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, MCF-7, SR, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SHANK3 antibody (orb1523478) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Detection of human SHANK3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SHANK3 antibody (orb1523478) used for IP at 6 µg per reaction.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001358973.1 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IP
Immunoprecipitation
Rabbit SHANK3 Antibody (orb1523478)
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