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Rabbit polyclonal antibodies to human IFN-gamma
SKU: orb2284549
Description
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | Western blot: 0.1-1.0 µg/ml |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG/IgM |
| Immunogen | Recombinant human IFN-Gamma (P01579) |
| Target | Interferon-gamma |
| Molecular Weight | 17 kDa |
| Purification | Immunogen affinity column |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Glycine pH 7.2 |
| Concentration | Batch dependent within range 2.0-2.5 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Western blot demonstrating our polyclonal detecting recombinant human Interferon-Gamma (24-166 of P01579) at 1 in 2000 dilution. Lane 1: Marker; Lane 2: IFN-γ (1ng); Lane 3: IFN-γ (5ng); Lane 4: IFN-γ (10ng); Lane 5: IFN-γ (50ng); Lane 6: Blank; Lane 7: IFN-γ (100ng); Lane 8: Blank; Lane 9: IFN-γ (200ng); Lane 10: Blank.
Recombinant Human Interferon gamma detected in a competition ELISA with monoclonal anti-Interferon gamma as the coating antibody, our polyclonal competes for binding to interferon gamma with a biotinylated polyclonal anti-human interferon gamma and detection is with Streptavidin-HRP.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000610.2 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Rabbit polyclonal antibodies to human IFN-gamma (orb2284549)
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