Rat Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit

SKU: orb777177

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-Site APP Cleaving Enzyme 1(bACE1).

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat bACE1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat bACE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat bACE1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat bACE1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	Serum, plasma, cerebrospinal fluid and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	15.63-1000 pg/mL
Sensitivity	6.7 pg/mL
Concentration	1000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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ASP2 ELISA Kit, Beta Secretase ELISA Kit, Memapsin 2 ELISA Kit, Aspartyl protease 2 ELISA Kit, Beta-site amyloid precursor protein cleaving enzyme 1 ELISA Kit, Membrane-associated aspartic protease 2 ELISA Kit

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Rat Beta-Site APP Cleaving Enzyme 1 (bACE1) ELISA Kit

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UniProt

P56819

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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