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Rat IgG Agarose Antibody
Description
Research Area
Images & Validation
−| Tested Applications | IP |
|---|---|
| Application Notes |
Key Properties
−| Source | Rat |
|---|---|
| Biological Origin | Rat |
| Isotype | IgG |
| Purity | This product is normal Rat IgG coupled to activated agarose. A single precipitin arc was observed against anti-Rat Serum when assayed by immunoelectrophoresis prior to coupling to the beads. Sufficient capacity is provided to bind a minimum of 10 mg of anti-Rat IgG. |
| Conjugation | Agarose |
Storage & Handling
−| Storage | Store vial at 4° C prior to opening. DO NOT FREEZE. |
|---|---|
| Form/Appearance | Suspension of agarose beads |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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This product is an purified Rat IgG isolated from normal Rat Serum digested by the enzyme pepsin and purified by chromatography coupled to activated agarose. Sufficient antibody capacity is provided to bind a minimum of 5.0 of anti-Rat IgG.
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SDS-PAGE of Rat IgG F(c) Fragment Rhodamine Conjugated. Lane M: 3 µl Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule (p/n orb2652744). Lane 2: Reduced Rat IgG F(c) Fragment Rhodamine Conjugated. Lane 3: Reduced Rat IgG Fab Fragment (p/n orb346340). Lane 4: Reduced Rat IgM Whole Molecule (p/n orb346341). Load: 1 µg of IgG, F(c), Fab; 1.5 µg of IgM. Predicted/Observed size: IgG at 55 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 78 and 25 kDa. Observed F(c) Fragment migrates slightly higher.
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Rat IgG Agarose Antibody (orb346335)
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