Rat muscle-specific RING-finger Protein 1 (MuRF1) ELISA Kit

SKU: orb1496442

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Description

Rat MuRF1 ELISA Kit

Research Area

Cell Biology

Images & Validation

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Item 1 of 1

Application Notes

standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MuRF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MuRF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MuRF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MuRF1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Rat
Sample Types	Serum, plasma, tissue homogenates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	15.63-1000 pg/mL
Sensitivity	6.6 pg/mL
Concentration	1000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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MuRF1 ; IRF ; RNF28 ; SMRZ ; TRIM63 ; IRFMURF2 ; Iris RING finger protein ; MuRF1 ; MURF1 ; MuRF-1 ; muscle specific ring finger protein 2 ; Muscle-specific RING finger protein 1 ; RING finger protein 28FLJ32380 ; RNF28E3 ubiquitin-protein ligase TRIM63 ; SMRZMURF-1 ; Striated muscle RING zinc finger protein ; tripartite motif containing 63 ; tripartite motif-containing 63 ; Tripartite motif-containing protein 63

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Rat muscle-specific RING-finger Protein 1 (MuRF1) ELISA Kit

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UniProt

Q91Z63

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No UniProt data available

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