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KRT77 Antibody

SKU: orb1274224

Description

KRT77 Antibody

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsIHC-P
ReactivityHuman
Application Notes
Immunohistochemistry (FFPE): 1-2 µg/mL for 30 min at RT (3)Prediluted IHC only format: incubate for 30 min at RT (2)Titering of the recombinant Acidic Cytokeratin antibody may be required for optimal performance.1. This antibody will detect cytokeratins -10, -14, -15, -16, -19.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min3. Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityRecombinant
IsotypeIgG, k
Clone No.KRTL/1577R
ImmunogenAn amino acid sequence common to acidic/type I keratins (1) was used as the immunogen for the recombinant Acidic Cytokeratin antibody.
TargetKRT77
PurificationProtein A affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Concentration0.2 mg/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

KRT77 Antibody

IHC staining of FFPE human lung carcinoma with recombinant Acidic Cytokeratin. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

UniProt Details

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol

KRT77 Antibody (orb1274224)

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100 μg
$ 1,140.00
DispatchUsually dispatched within 5-10 working days
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