Recombinant Bovine Hepatocyte growth factor (HGF) (Active)

SKU: orb3155913

Active

Description

Images & Validation

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Application Notes

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Key Properties

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Source	Mammalian cell
Biological Origin	Bos taurus (Bovine)
Biological Activity	①Measured by its binding ability in a functional ELISA. Immobilized Bovine HGF at 2 μg/mL can bind Anti-HGF recombinant antibody (CSB-RA010327MA1HU). The EC50 is 38.88-61.07 ng/mL. ②Measured by its binding ability in a functional ELISA.Immobilized Bovine HGF at 2 μg/mL can bind Human MET (CSB-MP012376MO1d9). The EC50 is 2.471-2.843 ng/mL. ③HGF Recombinant Monoclonal Antibody (CSB-RA010327MA1HU) captured on Protein A Chip can bind Bovine HGF with an affinity constant of 1.73 nM as detected by MetaSPR Assay (WeSPRTM 200). ④Recombinant Human MET (CSB-MP012376MO1d9) captured on Protein A Chip can bind Recombinant Bovine HGF with an affinity constant of 0.643 nM as detected by MetaSPR Assay (WeSPRTM 200).
Tag	C-terminal 10xHis-tagged
Molecular Weight	81.2 kDa
Expression Region	32-730aa
Protein Length	Full Length of Mature Protein
Protein Sequence	QKKRRNTLHEFKRSAKTTLIKEDPLLKIKTKKMNTADQCANRCIRNKGLPFTCKAFVFDKARKRCLWFPFNSMSSGVKKEFGHEFDLYENKDYIRNCIIGKGGSYKGTVSITKSGIKCQPWNSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGPMDHTETGKICQRWDHQTPHRHKFLPERYPDKGFDDNYCRNPDGKPRPWCYTLDPDTPWEYCAIKMCAHSTMNDTDLPMQTTECIQGQGEGYRGTINTIWNGIPCQRWDSQYPHQHDITPENFKCKDLRENYCRNPDGAESPWCFTTDPNIRVGYCSQIPKCDVSSGQDCYRGNGKNYMGSLSKTRSGLTCSMWDKNMEDLHRHIFWEPDATKLNKNYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTRTNVGWMVSLKYRNKHICGGSLIKESWILTARQCFPSRNKDLKDYEAWLGIHDVHGRGDEKRKQVLNVTQLVYGPEGSDLVLLKLARPAILDDFVSTIDLPNYGCTIPEKTTCSVYGWGYTGLINSDGLLRVAHLYIMGNEKCSQYHQGKVTLNESEICAGAENIVSGPCEGDYGGPLVCEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKIILTYKAPQL
Purity	Greater than 95% as determined by SDS-PAGE.
Endotoxins	Less than 1.0 EU/ug as determined by LAL method.

Storage & Handling

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Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Form/Appearance	Lyophilized powder
Buffer/Preservatives	Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Hepatocyte growth factor; Hepatopoietin-A; Scatter factor; SF [Cleaved into: Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain]

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

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UniProt

Q76BS1

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No UniProt data available

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