Recombinant CDKN1B Antibody / p27Kip1

SKU: orb3067110

Description

This MAb recognizes a 27kDa protein called p27Kip1/CDKN1B/Cyclin-dependent kinase inhibitor 1B, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb is excellent for staining of formalin-fixed tissues.

Research Area

Cancer Biology

Images & Validation

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Tested Applications	IHC-P, WB
Dilution Range	Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 2-4ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the recombinant CDKN1B antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG2a, kappa
Clone No.	rCDKN1B/13245
Immunogen	A recombinant human CDKN1B protein was used as the immunogen for the recombinant CDKN1B antibody.
Purification	Protein G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Quick Database Links

UniProt

P46527

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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