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Recombinant GM-CSF (Granulocyte-macrophage colony-stimulating factor), Mouse, Animal-Free
SKU: orb1178978
Description
Images & Validation
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| Tested Applications | Cell Culture, ELISA |
|---|
Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Origin | Mouse |
| Biological Activity | Measure by its ability to induce proliferation in FDC-P1 cells. The ED50 for this effect is <50 pg/mL. The specific activity of recombinant mouse GM-CSF is approximately >2 x 107 IU/mg. |
| Reactivity | Mouse |
| Tag | His-tag at the N-terminus |
| Protein Sequence | APTRSPITVTRPWKHVEAIKEALNLLDDMPVTLNEEVEVVSNEFSFKKLTCVQTRLKIFEQGLRGNFTKLKGALNMTASYYQTYCPPTPETDCETQVTTYADFIDSLKTFLTDIPFECKKPVQK with polyhistidine tag at the N-terminus. |
| Purity | >98% as determined by SDS-PAGE. Ni-NTA chromatography. |
| Endotoxins | <0.1 EU per 1 μg of the protein by the LAL method. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | The protein was lyophilized from a solution containing 1X PBS, pH 7.4. |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−colony stimulating factor 2, CSF2, CSF, Csfg, Csfgm, GMCS, GMCSF, Gm-CS, Gm-CSf, MGI-I, MGI-IGM
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Recombinant GM-CSF (Granulocyte-macrophage colony-stimulating factor), Mouse, Animal-Free (orb1178978)
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