Recombinant Human Glypican-3 (GPC3) (S359F), partial (Active)

SKU: orb1881655

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Description

This Recombinant Human Glypican-3 (GPC3)(S359F), partial (Active) spans the amino acid sequence from region 25-559aa(S359F). Purity: Greater than 90% as determined by SDS-PAGE.

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Application Notes	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Key Properties

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Source	Mammalian cell
Biological Origin	Homo sapiens (Human)
Biological Activity	Loaded Anti-Human GPC3 Antibody on 96-Flat plate, can bind Human GPC3 (S359F), with an affinity constant of 6.32 nM as determined in BLI assay (Gator Prime).
Tag	C-terminal 10xHis-tagged
Molecular Weight	62.2 kDa
Expression Region	25-559aa(S359F)
Protein Length	Partial
Protein Sequence	QPPPPPPDATCHQVRSFFQRLQPGLKWVPETPVPGSDLQVCLPKGPTCCSRKMEEKYQLTARLNMEQLLQSASMELKFLIIQNAAVFQEAFEIVVRHAKNYTNAMFKNNYPSLTPQAFEFVGEFFTDVSLYILGSDINVDDMVNELFDSLFPVIYTQLMNPGLPDSALDINECLRGARRDLKVFGNFPKLIMTQVSKSLQVTRIFLQALNLGIEVINTTDHLKFSKDCGRMLTRMWYCSYCQGLMMVKPCGGYCNVVMQGCMAGVVEIDKYWREYILSLEELVNGMYRIYDMENVLLGLFSTIHDSIQYVQKNAGKLTTTIGKLCAHSQQRQYRFAYYPEDLFIDKKVLKVAHVEHEETLSSRRRELIQKLKSFISFYSALPGYICSHSPVAENDTLCWNGQELVERYSQKAARNGMKNQFNLHELKMKGPEPVVSQIIDKLKHINQLLRTMSMPKGRVLDKNLDEEGFESGDCGDDEDECIGGSGDGMIKVKNQLRFLAELAYDLDVDDAPGNSQQATPKDNEISTFHNLGNVH
Purity	Greater than 90% as determined by SDS-PAGE.
Endotoxins	Less than 1.0 EU/μg as determined by LAL method.

Storage & Handling

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Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Form/Appearance	Lyophilized powder
Buffer/Preservatives	Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Disclaimer	For research use only

Alternative Names

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OCI5

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Recombinant Human Glypican-3 (GPC3) (S359F), partial (Active)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Loaded Anti-Human GPC3 Antibody on 96-Flat plate, can bind Human GPC3 (S359F), with an affinity constant of 6.32 nM as determined in BLI assay (Gator Prime).

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UniProt

P51654

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No UniProt data available

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