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Recombinant Human Interleukin-22
Description
Images & Validation
−Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by inducing IL-10 secretion of human COLO 205 cells is less than 0.3 ng/ml, corresponding to a specific activity of > 3.3 x106 IU/mg. |
| Molecular Weight | Approximately 33.6 kDa, non-disulfide-linked homodimeric protein containing of two 147 amino acid polypeptide chains. |
| Protein Sequence | MAPISSHCRL DKSNFQQPYI TNRTFMLAKE ASLADNNTDV RLIGEKLFHG VSMSERCYLM KQVLNFTLEE VLFPQSDRFQ PYMQEVVPFL ARLSNRLSTC HIEGDDLHIQ RNVQKLKDTV KKLGESGEIK AIGELDLLFM SLRNACI |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Endotoxins | Less than 1 EU/ug of rHuIL-22 as determined by LAL method. |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 5.0. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human IL-22 [orb2994340]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 43.4 KDa. Observed: 50-75 KDa, reducing conditions
50 μg, 500 μg, 1 mg, 10 μgHuman IL-22 [orb2994341]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 43.4 KDa. Observed: 50-62 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman IL-22 [orb2994085]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 17.7 KDa. Observed: 25-35 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman IL-22 [orb2994086]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 17.9 KDa. Observed: 25-35 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman IL-22 [orb2994578]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 16.9 KDa. Observed: 14 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg

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UniProt Details
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Protocol Information
Recombinant Human Interleukin-22 (orb1906326)
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