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Recombinant Human Interleukin-20
Description
Images & Validation
−Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human IL-20Rα and human IL-20Rβ co-transfected murine BaF3 pro-B cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x106 IU/mg. |
| Molecular Weight | Approximately 17.6 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids. |
| Protein Sequence | MLKTLNLGSC VIATNLQEIR NGFSEIRGSV QAKDGNIDIR ILRRTESLQD TKPANRCCLL RHLLRLYLDR VFKNYQTPDH YTLRKISSLA NSFLTIKKDL RLCHAHMTCH CGEEAMKKYS QILSHFEKLE PQAAVVKALG ELDILLQWME ETE |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Endotoxins | Less than 1 EU/ug of rHuIL-20 as determined by LAL method. |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.2, with trehalose. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−IL-20 Antibody [orb666995]
IF, IHC, WB
Human, Mouse
Rabbit
Polyclonal
Unconjugated
30 μl, 50 μl, 100 μl, 200 μlHuman IL-20 [orb3002259]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 17.7 KDa. Observed: 17 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgHuman IL-20RA [orb3002192]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 26.3 KDa. Observed: 35-50 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg

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UniProt Details
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Protocol Information
Recombinant Human Interleukin-20 (orb1906327)
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