Recombinant Human Leptin protein(LEP) (Active)

SKU: orb1650611

Active

Description

Images & Validation

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Key Properties

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Expression System	E.Coli
Biological Activity	Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human Leptin R transfected BaF3 murine proB cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 105 IU/mg.
Tag	NO-tagged
Molecular Weight	16.0 kDa
Protein Sequence	VPIQKVQDDT KTLIKTIVTR INDISHTQSV SSKQKVTGLD FIPGLHPILT LSKMDQTLAV YQQILTSMPS RNVIQISNDL ENLRDLLHVL AFSKSCHLPW ASGLETLDSL GGVLEASGYS TEVVALSRLQ GSLQDMLWQL DLSPGC
Purity	>97% as determined by SDS-PAGE and HPLC.
Endotoxins	Less than 1 EU/g of rHuLeptin as determined by LAL method.

Storage & Handling

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Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Form/Appearance	Lyophilized from a 0.2 m filtered concentrated solution in 50 mm PB, pH 3.5, with 0.02 % Tween-20.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Obese protein,

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UniProt

P41159

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No UniProt data available

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