Recombinant Human Liver carboxylesterase 1 (CES1) (Active)

SKU: orb2658694

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Description

This Recombinant Human Liver carboxylesterase 1 (CES1) spans the amino acid sequence from region 18-567aa. Purity: Greater than 95% as determined by SDS-PAGE.

Images & Validation

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Application Notes	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Key Properties

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Source	Mammalian cell
Biological Origin	Homo sapiens (Human)
Biological Activity	Measured by its ability to hydrolyze p-nitrophenylacetate. The specific activity is >40000 pmol/min/µg.
Tag	C-terminal 10xHis-tagged
Molecular Weight	62.0 kDa
Expression Region	18-567aa
Protein Length	Full Length of Mature Protein
Protein Sequence	GHPSSPPVVDTVHGKVLGKFVSLEGFAQPVAIFLGIPFAKPPLGPLRFTPPQPAEPWSFVKNATSYPPMCTQDPKAGQLLSELFTNRKENIPLKLSEDCLYLNIYTPADLTKKNRLPVMVWIHGGGLMVGAASTYDGLALAAHENVVVVTIQYRLGIWGFFSTGDEHSRGNWGHLDQVAALRWVQDNIASFGGNPGSVTIFGESAGGESVSVLVLSPLAKNLFHRAISESGVALTSVLVKKGDVKPLAEQIAITAGCKTTTSAVMVHCLRQKTEEELLETTLKMKFLSLDLQGDPRESQPLLGTVIDGMLLLKTPEELQAERNFHTVPYMVGINKQEFGWLIPMQLMSYPLSEGQLDQKTAMSLLWKSYPLVCIAKELIPEATEKYLGGTDDTVKKKDLFLDLIADVMFGVPSVIVARNHRDAGAPTYMYEFQYRPSFSSDMKPKTVIGDHGDELFSVFGAPFLKEGASEEEIRLSKMVMKFWANFARNGNPNGEGLPHWPEYNQKEGYLQIGANTQAAQKLKDKEVAFWTNLFAKKAVEKPPQTEHIEL
Purity	Greater than 95% as determined by SDS-PAGE. Greater than 95% as determined by SEC-HPLC.
Endotoxins	Less than 1.0 EU/μg as determined by LAL method.

Storage & Handling

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Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Form/Appearance	Lyophilized powder
Buffer/Preservatives	Lyophilized from a 0.2 μm sterile filtered 20 mM Tris-HCl, 0.5 M NaCl, 6%Trehalose, pH 8.0.
Disclaimer	For research use only

Alternative Names

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Liver carboxylesterase 1; Acyl-coenzyme A:cholesterol acyltransferase (ACAT); Brain carboxylesterase hBr1; Carboxylesterase 1 (CE-1; hCE-1) (EC:3.1.1.13 ) ; Cholesteryl ester hydrolase 1 publication (CEH 1 publication) (EC:3.1.1.134); Cocaine carboxylesterase;CES1 ；CES2, SES1

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Recombinant Human Liver carboxylesterase 1 (CES1) (Active)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

The purity of CES1 was greater than 95% as determined by SEC-HPLC

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UniProt

P23141

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No UniProt data available

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