Recombinant Human papillomavirus type 16 Protein E7 (E7) (Active)

SKU: orb1095874

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Description

This Recombinant Human papillomavirus type 16 Protein E7 (E7) (Active) spans the amino acid sequence from region 1-98aa. Purity: Greater than 90% as determined by SDS-PAGE.

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Application Notes	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Key Properties

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Source	E.coli
Biological Origin	Human papillomavirus type 16
Biological Activity	Measured by its binding ability in a functional ELISA. Immobilized HPV16 E7 at 10 μg/mL can bind Biotinylated MYC, the EC50 is 268.1-354.3 ng/mL
Tag	N-terminal 6xHis-tagged and C-terminal 6xHis-tagged
Molecular Weight	16.3 kDa
Expression Region	1-98aa
Protein Length	Full Length
Protein Sequence	MHGDTPTLHEYMLDLQPETTDLYCYEQLNDSSEEEDEIDGPAGQAEPDRAHYNIVTFCCKCDSTLRLCVQSTHVDIRTLEDLLMGTLGIVCPICSQKP
Purity	Greater than 90% as determined by SDS-PAGE.

Storage & Handling

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Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
Form/Appearance	Lyophilized powder
Buffer/Preservatives	Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Disclaimer	For research use only

Alternative Names

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Protein E7

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Recombinant Human papillomavirus type 16 Protein E7 (E7) (Active)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Measured by its binding ability in a functional ELISA. Immobilized HPV16 E7 at 10 μg/ml can bind Biotinylated MYC, the EC50 is 268.1-354.3 ng/mL.

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UniProt

P03129

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No UniProt data available

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