Recombinant Human Platelet-derived growth factor subunit B (PDGFB) (Active)

SKU: orb2657943

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Description

This Recombinant Human Platelet-derived growth factor subunit B (PDGFB) (Active) spans the amino acid sequence from region 82-190aa. Purity: Greater than 95% as determined by SDS-PAGE.

Images & Validation

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Application Notes	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Key Properties

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Source	Yeast
Biological Origin	Homo sapiens (Human)
Biological Activity	Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect is ≤5 ng/mL.
Tag	Tag free
Molecular Weight	12.2 kDa
Expression Region	82-190aa
Protein Length	Full Length of Mature Protein
Protein Sequence	SLGSLTIAEPAMIAECKTRTEVFEISRRLIDRTNANFLVWPPCVEVQRCSGCCNNRNVQCRPTQVQLRPVQVRKIEIVRKKPIFKKATVTLEDHLACKCETVAAARPVT
Purity	Greater than 95% as determined by SDS-PAGE.
Endotoxins	≤10 EU/mg by the LAL method

Storage & Handling

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Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Form/Appearance	Lyophilized powder
Buffer/Preservatives	Lyophilized from a 0.2 μm filtered solution containing 100mM Citratesodium citrate, 5% Mannitol 0.1% Trehalose pH 6.5
Disclaimer	For research use only

Alternative Names

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PDGFBB; PDGF-BB

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Quick Database Links

UniProt

P01127

UniProt Details

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No UniProt data available

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