Recombinant Macaca fascicularis sialic acid-binding Ig-like lectin 9 isoform X1 (SIGLEC9), partial (Active)

SKU: orb2986500

Active

Description

This Recombinant Macaca fascicularis (SIGLEC9), partial spans the amino acid sequence from region 20-350aa. Purity: Greater than 95% as determined by SDS-PAGE.

Images & Validation

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Application Notes

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Key Properties

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Source	Mammalian cell
Biological Origin	Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Biological Activity	①Measured by its binding ability in a functional ELISA.Immobilized Macaca fascicularis SIGLEC9 at 2 μg/mL can bind anti-SIGLEC9 recombinant antibody(CSB-RA021303MA2HU).The EC50 is 7.698-8.925 ng/mL. ②SIGLEC9 Recombinant Monoclonal Antibody(CSB-RA021303MA2HU) captured on Protein A Chip can bind Macaca fascicularis SIGLEC9 with an affinity constant of 4.34 nM as detected by MetaSPR Assay (WeSPRTM 200).
Tag	C-terminal 10xHis-tagged
Molecular Weight	37.5 kDa
Expression Region	20-350aa
Protein Length	Partial
Protein Sequence	QRNNQKNYPLTMQESVTVQQGLCVHVLCSFSYPWYGWISSDPVHGYWFRAGAHTDRDAPVATNNPARAVREDTRDRFHLLGDPQTKNCTLSIRDARSSDAGTYFFRVETGKTKWNYKYVPLSVHVTALTHRPNILIPGTLESGCPRNLTCSVPWACEQGTAPMISWMGTSVSPLDPSTTRSSVLTLIPQPQDHGTSLTCQVTFPGASVTTNKTIHLNVSYPPQNLTMTVFQGNDTVSIVLGNGSSVSVPEGPSLRLVCAVDSNPPARLSLSWGGLTLCPSQPSNPGVLELPRVHLRDEEEFTCRAQNLLGSQRVSLNVSLQSKATSGLTQG
Purity	Greater than 95% as determined by SDS-PAGE.

Storage & Handling

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Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Form/Appearance	Lyophilized powder
Buffer/Preservatives	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

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UniProt

XP_005590140.1

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