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Recombinant Macaca fascicularis IL23A&IL12B Heterodimer Protein (Active)
SKU: orb2992165
Active
Description
Research Area
Immunology & Inflammation
Images & Validation
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| Application Notes |
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Key Properties
−| Source | Mammalian cell |
|---|---|
| Biological Origin | Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) |
| Biological Activity | ①Measured by its binding ability in a functional ELISA.Immobilized Macaca fascicularis IL23A&IL12B at 2 μg/mL can bind anti-IL23A&IL12B recombinant antibody(CSB-RA011587MA2HU).The EC50 is 0.7723-0.9250 ng/mL. ②IL23A&IL12B Recombinant Monoclonal Antibody(CSB-RA011587MA2HU) captured on Protein A Chip can bind Macaca fascicularis IL23A&IL12B with an affinity constant of 0.27 nM as detected by MetaSPR Assay (WeSPRTM 200). |
| Tag | N-terminal 10xHis-tagged & C-terminal Twin-Strep-tagged |
| Molecular Weight | 22.1 kDa & 37.8 kDa |
| Expression Region | 22-189aa(IL23A) & 23-328aa(IL12B) |
| Protein Length | Heterodimer |
| Protein Sequence | VPGGSSPAWAQCQQLSQKLCTLAWSAHPLVGHMDLREEGDEETTNDVPHIQCGDGCDPQGLRDNSQFCLQRIRQGLIFYEKLLGSDIFTGEPSLLPDSPVGQLHASLLGLSQLLQPEGHHWETQQIPSPSPSQPWQRLLLRFKILRSLQAFVAVAARVFAHGAATLSP&IWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQSGEVLGSGKTLTIQVKEFGDAGQYTCHKGGEALSHSLLLLHKKEDGIWSTDVLKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSNPQGVTCGAVTLSAERVRGDNKEYEYSVECQEDSACPAAEERLPIEVMVDAIHKLKYENYTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCIQVQGKSKREKKDRIFTDKTSATVICRKNASFSVQAQDRYYSSSWSEWASVPCS |
| Purity | Greater than 95% as determined by SDS-PAGE. |
Storage & Handling
−| Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
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| Form/Appearance | Lyophilized powder |
| Buffer/Preservatives | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |

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Recombinant Macaca fascicularis IL23A&IL12B Heterodimer Protein (Active) (orb2992165)
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