Recombinant Macaca fascicularis Interleukin-2 receptor subunit alpha (IL2RA), partial (Active)

SKU: orb3155650

Active

Description

Images & Validation

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Application Notes

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Key Properties

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Source	Mammalian cell
Biological Origin	Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Biological Activity	①Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis IL2RA at 2 μg/mL can bind Macaca fascicularis IL2 (CSB-MP640916MOVd9). The EC50 is 88.60-116.8 ng/mL. ②Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis IL2RA at 2 μg/mL can bind Human IL2 (CSB-MP011629HU). The EC50 is 0.8151-1.087 ng/mL. ③Macaca fascicularis IL2 (CSB-MP640916MOVd9) captured on Protein A Chip can bind Recombinant Macaca fascicularis IL2RA with an affinity constant of 31.3 nM as detected by MetaSPR Assay (WeSPRTM 200).
Tag	C-terminal 10xHis-tagged
Molecular Weight	26.1 kDa
Expression Region	22-238aa
Protein Length	Partial
Protein Sequence	ELCDDDPPKITHATFKAVAYKEGTMLNCECKRGFRRIKSGSPYMLCTGNSSHSSWDNQCQCTSSAARNTTKQVTPQPEEQKERKTTEMQSQMQLADQVSLPGHCREPPPWENEATERIYHFVVGQTVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQLICTGETEPSQFPGEEEPQASPDGLPESETSRLVTTTDFRIQTEVAATMETFIFTTE
Purity	Greater than 95% as determined by SDS-PAGE. Greater than 95% as determined by SEC-HPLC.
Endotoxins	Less than 1.0 EU/ug as determined by LAL method.

Storage & Handling

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Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Form/Appearance	Lyophilized powder
Buffer/Preservatives	Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Interleukin-2 receptor subunit alpha

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UniProt

H6WS54

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No UniProt data available

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