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Description
Research Area
Cell Biology
Images & Validation
−
| Tested Applications | ELISA, IF, IHC |
|---|---|
| Dilution Range | ELISA 0.02 to 0.1 µg/ml; IF 1 to 20 µg/ml; IHC-C 5 µg/ml. Conformational antibody, not suitable for Western Blot application. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | mouse IgG2a |
| Clone No. | 2B8, chicken-mouse IgG2a chimeric antibody |
| Immunogen | Recombinant human MANF protein produced using CHO-based suspension cell line. Immunogen is purified from cell culture supernatant |
| Purification | MabSelect affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS pH 7.4, with 0.1% sodium azide |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ARMET, ARP
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Protocol Information
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Recombinant monoclonal antibody to human MANF (orb1108624)
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