Recombinant Rat Lymphocyte antigen 6 complex locus protein G6d (Ly6g6d) (Active)

SKU: orb1785093

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Description

This Recombinant Rat Lymphocyte antigen 6 complex locus protein G6d (Ly6g6d) (Active) spans the amino acid sequence from region 20-108aa. Purity: Greater than 90% as determined by SDS-PAGE.

Research Area

Immunology & Inflammation

Images & Validation

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Application Notes

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Key Properties

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Source	Yeast
Biological Origin	Rattus norvegicus (Rat)
Biological Activity	Measured by its binding ability in a functional ELISA. Immobilized Rat Ly6g6d at 5 μg/mL can bind Anti-LY6G6D recombinant antibody. The EC50 is 4.245-5.843 μg/mL.
Tag	N-terminal 6xHis-tagged
Molecular Weight	11.7 kDa
Expression Region	20-108aa
Protein Length	Full Length of Mature Protein
Protein Sequence	QRMRCYDCGGGPSNSCKQTVITCGEGERCGFLDRKPQPSSEQAKQPSATLSHHYPACVATHHCNQVAIESVGDVTFTTQKNCCFGDLCN
Purity	Greater than 90% as determined by SDS-PAGE.
Endotoxins	Less than 1.0 EU/μg as determined by LAL method.

Storage & Handling

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Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Form/Appearance	Lyophilized powder
Buffer/Preservatives	Lyophilized from a 0.2 μm sterile filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

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Recombinant Rat Lymphocyte antigen 6 complex locus protein G6d (Ly6g6d) (Active)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Measured by its binding ability in a functional ELISA. Immobilized Rat Ly6g6d at 2 μg/ml can bind Anti-Ly6g6d recombinant antibody. The EC50 is 6.238-7.258 ng/mL.

The purity of Ly6g6d was greater than 95% as determined by SEC-HPLC

Quick Database Links

UniProt

Q6MG58

UniProt Details

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No UniProt data available

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