RecombinantBetacellulin,Mouse

SKU: orb1494904

Description

Betacellulin is a pleiotropic cytokine that belongs to the Epidermal Growth Factor (EGF) family. Like other members of the EGF family, Betacellulin possesses a conserved sequence of 35-40 amino acids which contain 3 disulfide bonds formed by 6 cysteines. Betacellulin is unique in the EGF family since it can bind and activate a broad spectrum of ErbB receptors. Functionally, Betacellulin plays a role in the development of the pancreas by activating signaling pathways beneficial for the function, survival and regeneration of pancreatic β-cells. Additionally, Betacellulin has potential angiogenic activities and is important for the growth, development and repair of certain tissues.Recombinant mouse Betacellulin (rmBetacellulin) produced in E.coli is a single non-glycosylated polypeptide chain containing 81 amino acids. A fully biologically active molecule, rmBetacellulin has a molecular mass of 9.2 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Images & Validation

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Application Notes	Reconstituted in ddH2O at 100 μg/mL.

Key Properties

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Source	Escherichia coli.
Biological Activity	ED50 2× 10ˆ6 units/mg.
Molecular Weight	9.2 kDa, observed by reducing SDS-PAGE.
Protein Sequence	MDGNTTRTP ETNGSLCGAP GENCTGTTPR QKVKTHFSRC PKQYKHYCIH GRCRFVVDEQ TPSCICEKGY FGARCERVDL FY
Purification	> 95% by SDS-PAGE analysis.
Purity	> 95% by SDS-PAGE analysis.
Endotoxins	< 0.2 EU/μg, determined by LAL method.

Storage & Handling

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Storage	Lyophilized recombinant mouse Betacellulin (rmBetacellulin) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmBetacellulin remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Form/Appearance	Lyophilized after extensive dialysis against 50mM Tris, 300mM NaCl, pH 9.0.
Buffer/Preservatives	Lyophilized after extensive dialysis against 50mM Tris, 300mM NaCl, pH 9.0.
Disclaimer	For research use only

Alternative Names

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BTC

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