RecombinantCkbeta8‑1/CCL23,Human

SKU: orb1494757

Description

Chemokine (C-C motif) ligand 23 (CCL23) is a small cytokine belonging to the CC chemokine family that is also known as Macrophage inflammatory protein 3 (MIP-3) and Myeloid progenitor inhibitory factor 1 (MPIF-1). CCL23 is predominantly expressed in lung and liver tissue, but is also found in bone marrow and placenta.CCL23 is highly chemotactic for resting T cells and monocytes and slightly chemotactic for neutrophils. It has also been attributed to an inhibitory activity on hematopoietic progenitor cells. CCL23 is a ligand for the chemokine receptor CCR1.Recombinant human Ck beta 8‑1/CCL23 produced in CHO cells is a single polypeptide chain containing 92 amino acids. A fully biologically active molecule, rhCk beta 8‑1/CCL23 has a molecular mass of 10.5 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.

Images & Validation

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Application Notes	Reconstituted in ddH2O or PBS at 100 μg/ml.

Key Properties

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Source	CHO
Biological Activity	The EC50 value of human Ck beta 8‑1/CCL23 on Caˆ2+ mobilization assay in CHO-K1/Ga15/hCCR1 cells (human Ga15 and human CCR1 stably expressed in CHO-K1 cells) is less than 0.4 μg/ml.
Molecular Weight	10.5 kDa, observed by reducing SDS-PAGE.
Protein Sequence	MLWRRKIGPQMTLSHAAGFHATSADCCISYTPRSIPCSLLESYFETNSECSKPGVIFLTKKGRRFCANPSDKQVQVCVRM LKLDTRIKTRKN
Purification	> 98% as analyzed by SDS-PAGE.
Purity	> 98% as analyzed by SDS-PAGE.
Endotoxins	< 0.2 EU/μg, determined by LAL method.

Storage & Handling

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Storage	Lyophilized recombinant human Ck beta 8‑1°CCL23 remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, human Ck beta 8‑1°CCL23 should be stable up to 1 week at 4°C or up to 2 months at -20°C.
Form/Appearance	Lyophilized after extensive dialysis against PBS.
Buffer/Preservatives	Lyophilized after extensive dialysis against PBS.
Disclaimer	For research use only

Alternative Names

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CCL23

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