RecombinantFGF-10,His,Human

SKU: orb1494888

Description

Fibroblast Growth Factor-10 (FGF-10) is a mitogen mainly produced by mesenchymal stem cells in lung. FGF-10 belongs to the heparin binding FGF family, and is also known as Keratinocyte Growth Factor-2 (KGF-2). It shares the homolog and receptor FGFR2-IIIb with KGF. However, unlike KGF which induces the proliferation and differentiation of various epithelial cells, FGF-10 is an essential factor for the budding and branching morphogenesis during the multi-organ development via the instructive mesenchymal-epithelial interactions. FGF-10 is crucial for lung and limb development, and is regulated by Shh during early development. Recombinant human Fibroblast Growth Factor-10 (rhFGF-10) with N-terminal His-tag produced in E. coli is a single non-glycosylated polypeptide chain containing 187 amino acids. A fully biologically active molecule, rhFGF-10 has a molecular mass of 21.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Images & Validation

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Application Notes	Reconstituted in ddH2O at 100 μg/mL.

Key Properties

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Source	Escherichia coli.
Biological Activity	ED50 5.0× 10ˆ4 units/mg.
Molecular Weight	21.4 kDa, observed by reducing SDS-PAGE.
Protein Sequence	MNHKVHHHHHHMDDDDKMLG QDMVSPEATN SSSSSFSSPS SAGRHVRSYN HLQGDVRWRK LFSFTKYFLK IEKNGKVSGT KKENCPYSIL EITSVEIGVV AVKAINSNYY LAMNKKGKLY GSKEFNNDCK LKERIEENGY NTYASFNWQH NGRQMYVALN GKGAPRRGQK TRRKNTSAHF LPMVVHS
Purification	> 95% by SDS-PAGE analysis.
Purity	> 95% by SDS-PAGE analysis.
Endotoxins	< 0.2 EU/μg, determined by LAL method.

Storage & Handling

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Storage	Lyophilized recombinant human Fibroblast Growth Factor-10 (rhFGF-10) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhFGF-10 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Form/Appearance	Lyophilized after extensive dialysis against PBS.
Buffer/Preservatives	Lyophilized after extensive dialysis against PBS.
Disclaimer	For research use only

Alternative Names

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Fibroblast Growth Factor-10, FGFA, Keratinocyte growth factor-2

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