RecombinantIL-5,Human

SKU: orb1494817

Description

Interleukin-5 (IL-5), produced by mast cells, T cells and eosinophils, is responsible for the activities attributed to eosinophil differentiating factor, B cell growth factor II and T cell-replacing factor (TRF). It can increase production and mobilization of eosinophils and CD34+ progenitors from the bone marrow. IL-5 plays an important role in inducing cell-mediated immunity against parasitic infections and certain tumors. IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release.Recombinant Human Interleukin-5 (rhIL-5) produced in E.coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 116 amino acids each. A fully biologically active molecule, rhIL-5 has a molecular mass of 26.5 kDa analyzed by non-reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Images & Validation

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Application Notes	Reconstituted in ddH2O at 100 μg/ml.

Key Properties

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Source	Escherichia coli.
Biological Activity	ED50 1.0× 10ˆ6 units/mg.
Molecular Weight	26.5 kDa, observed by non-reducing SDS-PAGE.
Protein Sequence	MIPTEIPTS ALVKETLALL STHRTLLIAN ETLRIPVPVH KNHQLCTEEI FQGIGTLESQ TVQGGTVERL FKNLSLIKKY IDGQKKKCGE ERRRVNQFLD YLQEFLGVMN TEWIIES
Purification	> 95% by SDS-PAGE.
Purity	> 95% by SDS-PAGE.
Endotoxins	< 0.2 EU/μg, determined by LAL method.

Storage & Handling

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Storage	Lyophilized recombinant Human Interleukin-5 (rhIL-5) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIL-5 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Form/Appearance	Lyophilized after extensive dialysis against 25mM Tris, pH 8.0.
Buffer/Preservatives	Lyophilized after extensive dialysis against 25mM Tris, pH 8.0.
Disclaimer	For research use only

Alternative Names

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Interleukin-5, IL5

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