RecombinantNT-4,Human

SKU: orb1494786

Description

Neurotrophin-4 (NT-4), also known as NT-5, is a neurotrophic factor structurally related to β-NGF, BDNF, and NT-3. Human NT-4 shares 48 - 52% aa sequence identity with human β-NGF, BDNF, and NT-3.Neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds. NT-4 is expressed highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. NT-4 binds and induces receptor dimerization and activation of TrkB. NT-4 can signal through TrkB receptors and promotes the survival of peripheral sensory sympathetic neurons.Recombinant human Neurotrophin-4 (rhNT-4) produced in E.coli is a noncovalently linked homodimer containing two non-glycosylated polypeptide chains of 131 amino acids. A fully biologically active molecule, rhNT-4 has a molecular mass of 28.1kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Images & Validation

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Application Notes	Reconstituted in 50mM acetic acid or ddH2O at 50 μg/ml.

Key Properties

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Source	Escherichia coli.
Biological Activity	ED50 2.0× 10ˆ2 units/mg.
Molecular Weight	28.1 kDa, a noncovalently linked homodimer,of two 14.0 kDa polypeptide monomers.
Protein Sequence	MGVSETAPA SRRGELAVCD AVSGWVTDRR TAVDLRGREV EVLGEVPAAG GSPLRQYFFE TRCKADNAEE GGPGAGGGGC RGVDRRHWVS ECKAKQSYVR ALTADAQGRV GWRWIRIDTA CVCTLLSRTG RA
Purification	> 95% by SDS-PAGE and HPLC analyses.
Purity	> 95% by SDS-PAGE and HPLC analyses.
Endotoxins	< 0.3 EU/μg, determined by LAL method.

Storage & Handling

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Storage	Lyophilized recombinant human Neurotrophin-4 (rhNT-4) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhNT-4 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Form/Appearance	Lyophilized after extensive dialysis against 50mM acetic acid.
Buffer/Preservatives	Lyophilized after extensive dialysis against 50mM acetic acid.
Disclaimer	For research use only

Alternative Names

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Neurotrophin-4, Neurotrophic 4/5 (NT-4/NT-5)

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