RecombinantSCF,Human(P.pastoris-expressed)

SKU: orb1494611

Description

Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity during the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors.Recombinant human Stem Cell Factor (rhSCF) produced in Pichia pastoris is a glycosylated polypeptide chain of 165 amino acids. A fully biologically active molecule, rhSCF has a molecular mass of ~20kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.

Images & Validation

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Application Notes	Reconstituted in ddH2O at 100 μg/ml.

Key Properties

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Source	P. pastoris
Biological Activity	The ED50, as determined by the dose-dependent stimulation of human TF-1 cells, is < 2 ng/ml, corresponding to a specific activity of 5×10ˆ5 IU/mg.
Molecular Weight	~20 kDa, observed by reducing SDS-PAGE.
Protein Sequence	MEGICR NRVTNNVKDV TKLVANLPKD YMITLKYVPG MDVLPSHCWI SEMVVQLSDS LTDLLDKFSN ISEGLSNYSI IDKLVNIVDD LVECVKENSS KDLKKSFKSP EPRLFTPEEF FRIFNRSIDA FKDFVVASET SDCVVSSTLS PEKDSRVSVT KPFMLPPVA
Purification	> 95% by SDS-PAGE analysis.
Purity	> 95% by SDS-PAGE analysis.
Endotoxins	< 1.0 EU/μg, determined by LAL method.

Storage & Handling

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Storage	Lyophilized recombinant human Stem Cell Factor (rhSCF) remains stable up to 12 months at -80°C from date of receipt. Upon reconstitution, rhSCF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.
Form/Appearance	Lyophilized after extensive dialysis against 10 mM acetic acid.
Buffer/Preservatives	Lyophilized after extensive dialysis against 10 mM acetic acid.
Disclaimer	For research use only

Alternative Names

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C-Kit Ligand, Mast Cell Growth Factor (MGF), Steel Factor

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