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RecombinantSCF,Mouse

SKU: orb1494610

Description

Stem Cell Factor (SCF) is a hematopoietic growth factor that binds to the c-Kit receptor. SCF exerts its activity during the early stages of hematopoiesis. SCF stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. Recombinant mouse Stem Cell Factor (rmSCF) produced in P. pastoris is a single glycosylated polypeptide chain containing 165 amino acids. A fully biologically active molecule, rmSCF has a molecular mass of 18.4kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

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Application Notes
Reconstituted in ddH2O at 100 μg/ml.

Key Properties

SourceP. pastoris
Biological ActivityED50 1.0× 10ˆ5 units/mg.
Molecular Weight18.4kDa, observed by reducing SDS-PAGE.
Protein SequenceMKEICG NPVTDNVKDI TKLVANLPND YMITLNYVAG MDVLPSHCWL RDMVIQLSLS LTTLLDKFSN ISEGLSNYSI IDKLGKIVDD LVLCMEENAP KNIKESPKRP ETRSFTPEEF FSIFNRSIDA FKDFMVASDT SDCVLSSTLG PEKDSRVSVT KPFMLPPVA
Purification> 95% by SDS-PAGE and HPLC analyses.
Purity> 95% by SDS-PAGE and HPLC analyses.
Endotoxins< 0.2 EU/μg, determined by LAL method.

Storage & Handling

StorageLyophilized recombinant mouse Stem Cell Factor (rmSCF) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rmSCF should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Form/AppearanceLyophilized after extensive dialysis against 50mM Tris, pH 8.0.
Buffer/PreservativesLyophilized after extensive dialysis against 50mM Tris, pH 8.0.
DisclaimerFor research use only

Alternative Names

C-Kit Ligand, Mast Cell Growth Factor (MGF), Steel Factor
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RecombinantSCF,Mouse (orb1494610)

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Available Sizes

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10 μg
$ 180.00
50 μg
$ 450.00