RecombinantShh(C24II),Human

SKU: orb1494775

Description

Sonic Hedgehog (Shh) is a member of the Hedgehog (Hh) family of highly conserved proteins which are widely represented throughout the animal kingdom. In mammal, there are three related Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh). They share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93% identical). Sonic Hedgehog plays a role in cell growth, cell specialization, and the normal shaping (patterning) of the body. Shh is also important for development of the brain and spinal cord (central nervous system), eyes, limbs, and many other parts of the body.Recombinant human Sonic Hedgehog (C24II) (rhShh) produced in E.coli is a single non-glycosylated polypeptide chain containing 175 amino acids. A fully biologically active molecule, rhShh has a molecular mass of 19.7 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Images & Validation

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Application Notes	Reconstituted in ddH2O at 100 μg/ml.

Key Properties

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Source	Escherichia coli.
Biological Activity	ED50 500 units/mg.
Molecular Weight	19.7 kDa, observed by reducing SDS-PAGE.
Protein Sequence	IIG PGRGFGKRRH PKKLTPLAYK QFIPNVAEKT LGASGRYEGK ISRNSERFKE LTPNYNPDII FKDEENTGAD RLMTQRCKDK LNALAISVMN QWPGVKLRVT EGWDEDGHHS EESLHYEGRA VDITTSDRDR SKYGMLARLA VEAGFDWVYY ESKAHIHCSV KAENSVAAKS GG
Purification	> 95% by SDS-PAGE and HPLC analyses.
Purity	> 95% by SDS-PAGE and HPLC analyses.
Endotoxins	< 0.2 EU/μg, determined by LAL method.

Storage & Handling

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Storage	Lyophilized recombinant human Sonic Hedgehog (C24II) (rhShh) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhShh should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Form/Appearance	Lyophilized after extensive dialysis against PBS.
Buffer/Preservatives	Lyophilized after extensive dialysis against PBS.
Disclaimer	For research use only

Alternative Names

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Hhg1; Sonic Hedgehog

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