RecombinantTNF-α,Rat

SKU: orb1494606

Description

Tumor Necrosis Factor-Alpha (TNF-Alpha) also known as Cachectin and TNFSF1A, plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases; and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease.Recombinant rat Tumor Necrosis Factor-Alpha (rrTNF-Alpha) produced in P. pastoris is a glycosylated polypeptide chain containing 157 amino acids. A fully biologically active molecule, rrTNF-Alpha has a molecular mass of 17.4kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.

Images & Validation

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Application Notes	Reconstituted in ddH2O at 100 μg/ml.

Key Properties

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Source	P. pastoris
Biological Activity	ED50 2.0× 10ˆ7 units/mg.
Molecular Weight	17.4kDa, observed by reducing SDS-PAGE.
Protein Sequence	MLRSSS QNSSDKPVAH VVANHQAEEQ LEWLSQRANA LLANGMDLKD NQLVVPADGL YLIYSQVLFK GQGCPDYVLL THTVSRFAIS YQEKVSLLSA IKSPCPKDTP EGAELKPWYE PMYLGGVFQL EKGDLLSAEV NLPKYLDITE SGQVYFGVIA L
Purification	> 95% by SDS-PAGE and HPLC analyses.
Purity	> 95% by SDS-PAGE and HPLC analyses.
Endotoxins	< 0.2 EU/μg, determined by LAL method.

Storage & Handling

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Storage	Lyophilized recombinant rat Tumor Necrosis Factor-Alpha (rrTNF-Alpha) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rrTNF-Alpha should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Form/Appearance	Lyophilized after extensive dialysis against PBS.
Buffer/Preservatives	Lyophilized after extensive dialysis against PBS.
Disclaimer	For research use only

Alternative Names

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TNF-alpha, TNF-α; Tumornecrosis factor ligand super family member 2, TNF-a; TNFa, Cachectin, DIF, TNFA, TNFSF 2

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