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Reparixin

SKU: orb1303100

Description

Reparixin is a potent small molecule dual CXCR1/CXCR2 antagonist, exhibiting strong inhibition of CXCR1-mediated chemotaxis (IC50=1 nM) and weaker activity against CXCR2 (IC50=100 nM). It is widely used in research to investigate the role of IL-8 signaling in inflammation, cancer metastasis, and ischemia-reperfusion injury in both cellular and animal models.
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Research Area

Cell Biology, Immunology & Inflammation, Pharmacology & Drug Discovery

Images & Validation

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Reparixin

Key Properties

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CAS Number266359-83-5
MW283.39
Purity99.89% (May vary between batches)
FormulaC14H21NO3S
SMILESCC(C)Cc1ccc(cc1)[C@@H](C)C(=O)NS(C)(=O)=O
TargetCXCR
Solubility10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (7.06 mM);DMSO:50 mg/mL (176.44 mM)

Bioactivity

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Target IC50
CXCR1:1 nM|CXCR2:100 nM|CXCR1 (Ile43Val):80 nM (in L1.2 cells)|CXCR1 (wild-type):5.6 nM (in L1.2 cells)
Cell Research
L1.2 Cell suspension (1.5-3×106 cells/mL) is incubated at 37°C for 15 min in the presence of vehicle or of Reparixin (1 nM-1 μM) and next seeded in triplicates in the upper compartment of the chemotactic chamber. Different agonists are seeded in the lower compartment of the chamber at the following concentrations: 1 nM CXCL8, 0.03 nM fMLP, 10 nM CXCL1, 2.5 nM CCL2, 30 nM C5a. The chemotactic chamber is incubated at 37°C in air with 5% CO2 for 45 min (human PMNs) or 2 h (monocytes). At the end of incubation, the filter is removed, fixed, and stained and five oil immersion fields at high magnification (100×) are counted for each migration well after sample coding. L1.2 migration is evaluated using 5 μM pore size Transwell filters.
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Animal Research
C57BL/6J mice (8-10 weeks old/20-25 g) are used. The subcutaneous administration of Reparixin (30 mg/kg) is performed 60 minutes before cerebral ischemia induction. The animals are divided into the following three experimental groups: Sham (i.e., the group in which the arteries are visualized, but there is no occlusion of the middle cerebral artery), Vehicle (i.e., the group pre-treated with the vehicle, phosphate buffer solution, 60 minutes before MCAo) and Reparixin (i.e., the group pre-treated with the drug 60 minutes before MCAo). To evaluate neurological signs secondary to MCAo, the animals are assessed with the SHIRPA battery 24 h after reperfusion.
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Storage & Handling

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StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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DF 1681Y, CXCR2, CXCR1, CXC chemokine receptors, CXCR, inhibit, Inhibitor, Repertaxin, Reparixin

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Compound Identifiers

PubChem CID
9838712 ↗

Key Properties

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No computed properties available.

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Reparixin (orb1303100)

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% DMSO +
%+
% Tween 80 +
%

Available Sizes

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5 mg
$ 80.00
10 mg
$ 100.00
1 ml x 10 mM (in DMSO)
$ 160.00
25 mg
$ 170.00
50 mg
$ 240.00
100 mg
$ 380.00
200 mg
$ 540.00
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