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SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein
SKU: orb1231613
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Description
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA.Immobilized Recombinant Human ACE2 at 2 g/mL (100L/well ) can bind Recombinant nCoV-S1, The EC50 of nCoV-S1 is 0.25-0.45 μg/mL |
| Target | S |
| Tag | His tag at C-terminus |
| Molecular Weight | 75.8 kDa |
| Protein Sequence | Val16-Arg685 |
| Purity | > 90% as determined by SDS-PAGE; > 95% by HPLC. |
Storage & Handling
−| Storage | Store the lyophilized protein at -20˚°C to -20˚°C for long term. After reconstitution, the protein solution is stable at -20˚°C for 3 months, at 2-8˚°C for up to 1 week |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Disclaimer | For research use only |
Alternative Names
−S1 protein, Spike glycoprotein subunit 1, S glycoprotein subunit 1, Spike protein S1, Novel coronavirus S1 protein
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Immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 at 2μg/mL (100 μL/well) can bind recombinant human ACE2 with a linear range of 0.5-8.7 ng/mL.
The purity of SARS-CoV-2 (COVID-19) Spike S1 recombinant protein was greater than 95% as determined by SEC-HPLC.
SARS-CoV-2 (COVID-19) spike S1 recombinant protein on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| RefSeq | YP_009724390.1 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SARS-CoV-2 (COVID-19) Spike S1 Recombinant Protein (orb1231613)
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