Recombinant SARS-Cov-2 Spike RBD protein (K417N, L452R,T478K), His (HEK293)

SKU: orb1516632

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Description

Recombinant SARS-Cov-2 Spike RBD protein (K417N, L452R,T478K), His (HEK293), with a concentration of >0.5 mg/ml, expressed in HEK293 cell, with a molecular weight of 36 kDa, and a purity of >90% as determined by SDS-PAGE. It was purified by AC, and has a His tag.

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Application Notes	Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Key Properties

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Expression System	HEK293 cell
Target	Spike glycoprotein
Tag	His
Molecular Weight	36 kDa
Protein Sequence	319-541/1273
Purification	AC
Purity	>90% as determined by SDS-PAGE
Endotoxins	Not analyzed

Storage & Handling

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Storage	Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles.
Concentration	>0.5 mg/ml
Disclaimer	For research use only

Alternative Names

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SARS-CoV-2 spike protein; 2019-nCOV Spike protein; Spike glycoprotein; Spike protein S1; Surface glycoprotein; Spike protein RBD; SPIKE_SARS2; 2019-nCOV Spike protein(K417N,L452R,T478K), Recombinant (His Tag); Recombinant SARS-COV-2 S protein RBD (K417N,L452R,T478K) Protein. ;Recombinant SARS-Cov-2 Spike RBD protein (K417N, L452R,T478K), His (HEK293)

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Recombinant SARS-Cov-2 Spike RBD protein (K417N, L452R,T478K), His (HEK293)

Measured by its binding ability in a functional ELISA. Immobilized human ACE2, His-Avi Tag (Cat: orb1516578) at 2 µg/ml (100 ul/Well) can bind SARS-CoV-2 Spike RBD (L452Q, F490S), His Tag (Cat: orb1516632), the EC50 is 74 ng/ml.

The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.

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Gene Symbol

Spike glycoprotein

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