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Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, WB |
|---|---|
| Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mSDF-1alpha (murine SDF-1alpha). |
| Target | Cxcl12 |
| Purification | Anti-mSDF-1alpha specific antibody was purified by affinity chromatography and then biotinylated. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | SDF-1a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Pbsf, Sdf1, Tlsf, Tpar1, Scyb12, Stromal cell-derived factor 1, 12-O-tetradecanoylphorbol 13-acetate repressed protein 1, SDF-1
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To detect Murine SDF-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Murine SDF-1-alpha (orb1272314) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Murine SDF-1-alpha.
To detect Murine SDF-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine SDF-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect Murine SDF-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine SDF-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Cxcl12 Antibody (Biotin) (orb1272313)
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