Recombinant SDHB Antibody / Succinate dehydrogenase B

SKU: orb1824555

Description

Succinate dehydrogenase B (SDHB) is an iron-sulfur subunit of mitochondrial complex II, a key component of the citric acid cycle and the electron transport chain, a respiratory complex that catalyzes the oxidation of succinate in the mitochondrial membrane.Many cancers are generally positive for SDHB, including renal cell carcinomas and gastrointestinal stromal tumors. A subset of RCC and GIST tumors that are associated with SDH mutations, Carney-Stratakis Syndrome or Carney Triad exhibit a loss of SDHB expression.

Images & Validation

−

Item 1 of 1

Tested Applications	IHC-P
Dilution Range	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Reactivity	Human
Application Notes	Optimal dilution of the recombinant SDHB antibody should be determined by the researcher.

Key Properties

−

Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG1, kappa
Clone No.	rSDHB/6948
Immunogen	A recombinant partial protein sequence (within amino acids 180-280) from the human protein was used as the immunogen for the recombinant SDHB antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Recombinant SDHB Antibody / Succinate dehydrogenase B

IHC staining of FFPE human hepatocellular carcinoma tissue with recombinant SDHB antibody (clone rSDHB/6948). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

P21912

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

IHC-P

Immunohistochemistry Paraffin

View Protocol

Find More Protocols