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SGK3 Antibody (N-term)
SKU: orb1928742
Description
Research Area
Metabolism Research, Signal Transduction
Images & Validation
−Item 1 of 4
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution Range | WB - 1:1000, IHC-P - 1:100-500 |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 57108 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CISK, SGKL
Quality Guarantee
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Western blot analysis of anti-SKG3 Pab in A375 cell lysate. SGK3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with SGK3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
IHCdetection of SGK3 protein on the paraffin sections of the WT (left) and YPC (right) mice at P0 (B and C), P3 (D and E), and P5 (F and G) skin. Positive signals were observed in the cytoplasm of the hair follicle keratinocytes, especially in hair bulb, ORS, IRS, cuticle/cortex and bulge, or sebaceous glands. Some differences between the WT and YPC, for example, the expression in bulb keratinocytes were observed at P3 and P5. Scale bar, 50.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
SGK3 Antibody (N-term) (orb1928742)
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