Smad3 Rabbit Polyclonal Antibody

SKU: orb704231

Description

Research Area

Cancer Research, Cell Biology, Cell Signaling, Cytoplasmic, Hypoxia, Intracellular Signaling Proteins, SMAD Family

Images & Validation

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Item 1 of 2

Tested Applications	WB
Dilution range	WB=1:500-2000
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Equine, Porcine, Sheep

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Smad3 (61-160/425aa)
Target	SMAD3
Molecular Weight	47 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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hMAD 3; hSMAD3; HSPC193; JV15 2; JV152; MAD(mothers against decapentaplegic Drosophila) homolog 3; MAD3; MADH 3; MADH3; Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 3; SMA and MAD related protein 3; SMAD 3; SMAD; SMAD-3; SMAD3_HUMAN.

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Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Heart tissue lysates, Primary: Anti-Smad3 (orb704231) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kDa, Observed band size: 47 kDa.

Sample: A431 (Human) Cell Lysate at 30 ug, Primary: Anti-Smad3 (orb704231) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 47 kD.

Quick Database Links

Gene Symbol

SMAD3

UniProt

P84022

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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