Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG
Clone No.	B7J33
Immunogen	KLH conjugated synthetic peptide derived from human SMAD7
Target	SMAD7
Molecular Weight	46 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Madh7; SMAD7_HUMAN; SMAD7; MAD homolog 7; Mothers against DPP homolog 7; Mothers against decapentaplegic homolog 8 (MAD homolog 8 | Mothers against DPP homolog 8); SMAD family member 7 (SMAD 7 | Smad7 | hSMAD7); MADH8; SMAD7_MOUSE; SMAD family member 7 (SMAD 7 | Smad7); SMAD7_RAT;

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Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SMAD7) Monoclonal Antibody, Unconjugated (orb1152081) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SMAD7) Monoclonal Antibody, Unconjugated (orb1152081) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (SMAD7) Monoclonal Antibody, Unconjugated (orb1152081) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: Human Hela cell Lysates, Lane 2: Human 293T cell Lysates, Primary: Anti-SMAD7 (orb1152081) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 46kDa, Observed band size: 46kDa.

Quick Database Links

Gene Symbol

SMAD7

UniProt

O15105

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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