SN-38

SKU: orb1307686

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Description

SN-38 (NK012) is the active metabolite of the Topoisomerase I inhibitor Irinotecan. It potently inhibits DNA/RNA synthesis and exhibits antitumor activity, including the induction of autophagy, making it a valuable tool for in vitro and in vivo cancer research.

Research Area

Cell Biology, Metabolism Research, Molecular Biology

Images & Validation

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Key Properties

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CAS Number	86639-52-3
MW	392.41
Purity	>98%
Formula	C₂₂H₂₀N₂O₅
SMILES	C(C)C1=C2C(C=3N(C2)C(=O)C4=C(C3)[C@](CC)(O)C(=O)OC4)=NC=5C1=CC(O)=CC5
Target	Topoisomerase
Solubility	Soluble in DMSO (up to 40 mg/ml).

Bioactivity

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Target IC50	RNA synthesis:1.3 μM\|CLM22 organoids:10.67 nM\|A549 cells proliferation:200.0 ± 14.9 ng/mL\|CWH22 organoids:7.54 nM\|MCF-7 cells proliferation:16.0 ± 0.7 ng/mL\|DNA synthesis:0.077 μM Read more...
In Vivo	METHODS: To test the antitumor activity in vivo, SN-38 (2 mg/kg) was administered by single intraperitoneal injection to C57BL/6 mice transplanted with LLC cells in the peritoneal cavity. RESULTS: A single intraperitoneal injection of SN-38 significantly attenuated the growth of LLC tumors, resulting in a 22.7% reduction in tumor growth. METHODS: To test the antitumor activity in vivo, SN-38 (10 mg/kg in 0.5% carboxymethylcellulose sodium, intraperitoneal injection) and gefitinib (100 mg/kg, subcutaneous injection) were administered to BALB/c nude mice bearing human oral squamous tumors HSC-2 five times per week for three weeks. RESULTS: Only gefitinib was administered to mice with human oral squamous tumors. RESULTS: There was no significant difference in tumor growth inhibition between gefitinib only and gefitinib plus SN-38 treatment. However, some tumors in the gefitinib-only group showed new growth when tumor measurements were continued after treatment was stopped. Read more...
In Vitro	METHODS: Lung cancer cells LLC, A549 and H358 were treated with SN-38 (10-1000 nM) for 48 h. Cell viability was detected using MTT assay. RESULTS: SN-38 started to exhibit effects at 10 nM concentration and induced about 50% cell death at 100 nM. METHODS: Colorectal cancer cells KM12C, KM12SM and KM12L4a were treated with SN-38 (2.5 µg/mL) for 4-48 h. Cell cycle and apoptosis were detected by Flow cytometry. RESULTS: SN-38 induced S-phase and G2-phase block, with KM12L4a cells responding most strongly in a time-dependent manner. apoptosis increased over time in the KM12SM and KM12L4a cell lines, but there was no such change in the KM12C cells. Read more...
Cell Research	MTT assay(Only for Reference)

Storage & Handling

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Storage	-20°C
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ADC Payload, ADC Cytotoxin, ADCCytotoxin, Autophagy, DNA synthesis, DNA Synthesis, DNASynthesis, Inhibitor, inhibit, NK012, NK-012, NK 012, Topoisomerase, Topo I, RNA Synthesis, RNASynthesis, SN 38, SN38, SN-38

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Compound Identifiers

PubChem CID

104842 ↗

Key Properties

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No computed properties available.

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SN-38 (orb1307686)

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Dynamic Range:	78.13 - 5,000 ng/mL
Sensitivity:	40.3 ng/mL

Dynamic Range:	156.25 - 10,000 ng/mL
Sensitivity:	53.67 ng/mL

Purity:	0.98
CAS Number:	1279680-68-0
MW:	1738.496
Formula:	C₇₇H₁₀₁CL₄N₁₁O₂₆

Purity:	>98%
CAS Number:	2400904-71-2
MW:	589.549488306046
Formula:	C₃₀H₂₇N₃O₁₀

CAS Number:	3025787-76-9
MW:	711.76
Formula:	C₃₈H₄₁N₅O₉

SN-38

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

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SN-38 (orb1307686)

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