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SPARC rabbit pAb Antibody
SKU: orb767126
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Description
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−Item 1 of 2
| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution range | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications. |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human SPARC. AA range:141-190 |
| Molecular Weight | 34 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-SPARC antibody, anti-ON antibody, anti-SPARC antibody, anti-Basement-membrane protein 40 antibody, anti-BM-40 antibody, anti-Osteonectin antibody, anti-ON antibody, anti-Secreted protein acidic and rich in cysteine antibody
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Western Blot analysis of HeLa, HepG2 cells using SPARC Polyclonal Antibody. Secondary antibody was diluted at 1:20000.
Immunohistochemical analysis of paraffin-embedded mouse-muscle, antibody was diluted at 1:100.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SPARC rabbit pAb Antibody (orb767126)
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