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SPINT1 Antibody
SKU: orb1257029
Featured
Description
Research Area
Cancer Biology, Cell Biology, Immunology & Inflammation
Images & Validation
−Item 1 of 4
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 234-513 of human SPINT1 (NP_003701.1). |
| Target | SPINT1 |
| Molecular Weight | Observed: 70kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−Kunitz-type protease inhibitor 1, Hepatocyte growth factor activator inhibitor type 1, HAI-1, SPINT1, HAI1
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SPINT1 Antibody [orb631469]
ELISA, IF, IHC, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μg, 50 μg
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Western blot analysis of extracts of various cell lines, using SPINT1 antibody (orb1257029) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human prostate using SPINT1 antibody (orb1257029) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse esophageal cancer using SPINT1 antibody (orb1257029) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using SPINT1 antibody (orb1257029). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
SPINT1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
SPINT1 Antibody (orb1257029)
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