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SRC Antibody

SKU: orb1261172

Description

SRC Antibody

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

Tested ApplicationsIHC, WB
ReactivityHuman, Mouse, Rat
Application Notes
WB: 1:500 - 1:2000IHC: 1:50 - 1:200

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human SRC (NP_005408.1).
TargetSRC
Molecular WeightObserved: 60kDa
PurificationAffinity purification
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Concentrationbatch dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

SRC, ASV, Pp60c-src, Tyrosine kinase pp60c-src, Tyrosine-protein kinase SRC-1, v-src, C-SRC, p60-Src, Proto-oncogene c-Src, SRC1

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SRC Antibody

Western blot analysis of extracts of various cell lines, using SRC antibody (orb1261172) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

SRC Antibody

Immunohistochemistry of paraffin-embedded human lung cancer using SRC antibody (orb1261172) at dilution of 1:100 (40x lens).

SRC Antibody

Immunohistochemistry of paraffin-embedded human breast cancer using SRC antibody (orb1261172) at dilution of 1:100 (40x lens).

SRC Antibody

Immunohistochemistry of paraffin-embedded human placenta using SRC antibody (orb1261172) at dilution of 1:100 (40x lens).

SRC Antibody

Immunohistochemistry of paraffin-embedded human gastric cancer using SRC antibody (orb1261172) at dilution of 1:100 (40x lens).

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol

SRC Antibody (orb1261172)

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Available Sizes

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100 μl
$ 830.00
DispatchUsually dispatched within 5-10 working days
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