Sulfasalazine

SKU: orb1310181

Description

Sulfasalazine is a prodrug derived from salicylic acid that exhibits affinity for elastin-rich tissues. It is widely used in research to study ferroptosis and inflammatory pathways, as it inhibits NF-κB, TGF-β, and COX-2 in both cellular and animal models of disease.

Research Area

Cell Biology, Immunology & Inflammation, Infectious Disease & Virology, Neuroscience, Signal Transduction

Images & Validation

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Key Properties

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CAS Number	599-79-1
MW	398.39
Purity	99.28% (May vary between batches)
Formula	C₁₈H₁₄N₄O₅S
SMILES	OC(=O)C1=CC(=CC=C1O)\N=N\C1=CC=C(C=C1)S(=O)(=O)NC1=CC=CC=N1
Target	Autophagy,Antibacterial,COX,Ferroptosis,Antibiotic,NF-κB,Apoptosis
Solubility	DMSO:200 mg/mL (502.02 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:20 mg/mL (50.2 mM);NaOH:250 mg/mL (627.53 mM)

Bioactivity

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Target IC50	Synthesis of leukotriene B4:250 μM\|PAF:50 µM\|Cystine uptake:24.7 ± 5.6 μM\|Synthesis of 5-hydroxyeicosatetraenoic acid:250 μM\|contractile leukotriene activity:0.15 mM\|Synthesis of leukotriene C4:100 μM\|synthesis of LTB4:2.8 mM (endogenous arachidonic acid is the substrate)\|synthesis of LTB4:0.8 mM (exogenous arachidonic acid is the substrate) Read more...
In Vivo	METHODS: To detect antitumor activity in vivo, Sulfasalazine (250 mg/kg) was administered intraperitoneally to C57BL/6N mice bearing B16F10 xenografts once daily for three days. Twenty-four hours after the third Sulfasalazine dose, local X-ray irradiation was applied to anesthetized tumor-bearing C57BL/6N mice at a dose of 4 Gy. RESULTS: Sulfasalazine alone did not significantly inhibit tumor growth; X-ray irradiation partially reduced tumor growth; the combination of Sulfasalazine and X-rays synergistically reduced tumor growth. Read more...
In Vitro	METHODS: Rat glioma cells F98 and human glioma cells U251 were treated with Sulfasalazine (200-400 µM) for 96 h. Cell viability was measured by MTT assay. RESULTS: Cell viability was significantly reduced in F98 at 200-400 µM Sulfasalazine concentration and U251 showed reduced cell viability at 400 µM Sulfasalazine. METHODS: Mouse melanoma cells B16F10 and mouse embryonic fibroblasts MEF were treated with Sulfasalazine (10-1000 µM) for 24 h. Cellular ROS levels were measured by DCFDA staining. RESULTS: At lower Sulfasalazine concentrations (10-100 µM), no increase in intracellular ROS was observed. At higher concentrations of Sulfasalazine (800-1000 µM), there was an approximately 2.3-fold increase in intracellular ROS in B16F10 cells, while no increase in ROS was observed in MEF cells. Read more...
Cell Research	Sulfasalazine is dissolved in culture medium. SW620 cells are grown in Dulbecco's modified Eagle medium, supplemented with 10% heat-inactivated FCS, 2 mmol/liter glutamine, and 1% (wt/vol) penicillin/streptomycin. SW620 cells are transfected with the 3xIgkBLuc reporter construct. After 18 h, cells are incubated with either medium alone or with sulfasalazine (0.1, 0.2, 0.5, 1, 2, 5 mM) before stimulation with TNFα, LPS, or PMA. Luciferase assay is performed. Read more...

Storage & Handling

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Storage	keep away from direct sunlight,keep away from moisture \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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COX-2, COX-1, Apoptosis, Antibiotic, Bacterial, Azulfidine, Autophagy, NSC 667219, inhibit, Nuclear factor-κB, Nuclear factor-kappaB, NSC667219, NSC-667219, NF-κB, NFκB, NFkB, NF-kB, Ferroptosis, Inhibitor, Sulfasalazine

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Compound Identifiers

PubChem CID

5339 ↗

Key Properties

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No computed properties available.

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Sulfasalazine (orb1310181)

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CAS Number:	1346601-18-0
MW:	192.62
Formula:	C₇D₃H₅ClNO₃

CAS Number:	1346606-50-5
MW:	402.42
Formula:	C₁₈H₁₀D₄N₄O₅S

Purity:	99.85%
CAS Number:	144-83-2
MW:	249.29
Formula:	C₁₁H₁₁N₃O₂S

CAS Number:	144-83-2
MW:	249.29
Formula:	C₁₁H₁₁N₃O₂S

Sulfasalazine

Description

Research Area

Images & Validation

Key Properties

Bioactivity

Storage & Handling

Alternative Names

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