TARDBP Antibody (N-term)

Western blot analysis of lysates from Hela cell line, mouse brain tissue lysate (from left to right), using TARDBP Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

TARDBP Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of TARDBP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Fluorescent confocal image of Hela cell stained with TARDBP Antibody (N-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TARDBP primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).TARDBP immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.