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Tau (K18) P301L Mutant Pre-formed Fibrils
SKU: orb1822356
Featured
Description
Research Area
Cell Biology, Disease Biomarkers, Neuroscience, Signal Transduction
Images & Validation
−Item 1 of 4
| Tested Applications | In vitro, In vivo, SDS-PAGE, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | Recombinant |
|---|---|
| Expression System | E. coli |
| Biological Origin | Human |
| Biological Activity | Thioflavin T emission curve shows increased fluorescence (correlated to tau protein fibrillation) when active tau PFFs are combined with active tau monomers. |
| Target | Tau (K18) P301L PFFs |
| Reactivity | Human |
| Tag | No tag |
| Molecular Weight | ~15.1 kDa |
| Protein Length | Partial |
| Protein Sequence | SRLQTAPVPM PDLKNVKSKI GSTENLKHQP GGGKVQIINK KLDLSNVQSK CGSKDNIKHVLGGGSVQIVY KPVDLSKVTS KCGSLGNIHH KPGGGQVEVK SEKLDFKDRV QSKIGSLDNI THVPGGGNKK IETHKLTFRE |
| Purification | Ion-exchange Purified |
| Purity | >95% |
Storage & Handling
−| Storage | -80°C |
|---|---|
| Buffer/Preservatives | 10 mM HEPES, 100 mM NaCl pH 7.4 |
| Concentration | 2 mg/ml |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−Tau PFFs, Tau PFF, Tau protein Pre-formed Fibrils, Tau aggregates, microtubule-associated protein Tau, MAPT, MAP, microtubule-associated protein, Paired Helical Filament-Tau, Phf-Tau, Neurofibrillary Tangle Protein, G Protein Beta1/Gamma2 Subunit-Interacting Factor 1, Isoform 4, tubulin-associated unit
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TEM of recombinant Tau (K18), P301L mutant Pre-formed Fibrils (PFFs) at 150kx magnification. HV=80kV.
TEM of recombinant Tau (K18), P301L mutant Pre-formed Fibrils (PFFs) at 150kx magnification. HV=80kV.
Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time in tau Pre-formed Fibrils. Tau Pre-formed Fibrils seed the formation of new tau fibrils when combined with tau monomers. Thioflavin T ex = 450 nm, em = 485 nm. 10 uM heparin was added to each well.
SDS-PAGE of ~15 kDa Active Human Tau Protein K18 P301L Pre-formed Fibrils. Lane 1: MW Ladder. Lane 2: Tau Protein Pre-formed Fibrils.
Quick Database Links
Gene Symbol
Tau (K18) P301L PFFs
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
Tau (K18) P301L Mutant Pre-formed Fibrils (orb1822356)
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