Recombinant Tenascin C Antibody

SKU: orb2642145

Description

Tenascin C is a multifunctional, disulfide-linked hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.

Images & Validation

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Tested Applications	IHC-P
Dilution Range	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Reactivity	Human
Application Notes	Optimal dilution of the recombinant Tenascin C antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	TNC/2981R
Immunogen	Recombinant human protein was used as the immunogen for the recombinant Tenascin C antibody.
Purification	Protein A affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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IHC staining of FFPE human lung carcinoma with recombinant Tenascin C antibody (clone TNC/2981R). HIER: boil tissue sections in 10 mM Tris with 1 mM EDTA, pH9, for 10-20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Tenascin C antibody (clone TNC/2981R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P24821

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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