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TGFa Antibody

SKU: orb749808

Description

This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this mAb is completely blocked by the peptide used for raising this antibody. TGFa is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.

Research Area

Epigenetics

Images & Validation

Tested ApplicationsFACS, IF, IHC-P
Dilution RangeFlow cytometry: 0.5-1ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
ReactivityHuman
Application Notes
Optimal dilution of the TGFa antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG1, kappa
Clone No.TG86
ImmunogenAmino acids 34-43 (PPVAAAVVSH) from the human protein were used as the immunogen for the TGFa antibody.
PurificationProtein G affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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TGFa Antibody

IHC: Formalin-fixed, paraffin-embedded human testicular carcinoma stained with TGFa antibody (TG86)

UniProt Details

No UniProt data available

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
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IF
Immunofluorescence
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TGFa Antibody (orb749808)

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20 μg
$ 370.00
100 μg
$ 750.00
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