Thermolabile UNG (Uracil N-Glycosylase) - 1 u/μl

SKU: orb3032251

Description

Thermolabile UNG is used in real-time PCR to prevent carry-over contamination of dU-containing DNA from previous reactions. Uracyl N-Glycosylase (UNG, UDG) catalyses the release of uracil from single and double stranded uracyl-containing DNA. The resulting abasic sites are susceptible to hydrolytic cleavage at elevated temperatures.\nAn amount of 1 unit UNG completely digests 104 to 106 copies of U-containing DNA fragments in 2 min at 50°C.

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Key Properties

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Storage & Handling

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Storage	store at -20 °C. avoid freeze/thaw cycles
Form/Appearance	liquid (Supplied in 20 mM Tris-HCl pH 8.0, 50 mM NaCl,1 mM EDTA, 1 mM DTT, 100 μg/ml rAlbumin and 50 % [v/v] glycerol)
Concentration	1 unit/μl
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Prevention of carry-over contaminations, UNG, UDG

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