Recombinant TIMP1 Antibody

SKU: orb639508

Description

TIMP-1, TIMP-2, TIMP-3 and TIMP-4 (for tissue inhibitor of metalloproteinases -1, -2, -3 and -4) complex with metalloproteinases such as collagenases, gelatinases and stromelysins, resulting in irreversible inactivation of the metalloproteinase. TIMP-1 is identical to EPA (erythroid-potentiation activity). PTH has been shown to be a regulator of TIMP-2 in osteoblastic cells. TIMP-3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes. TIMP-4 is most highly expressed in heart tissues. Studies have demonstrated that TIMP1 is useful as a biomarker for early detection of colorectal cancer, outperforming CEA. Additionally, TIMP1 studies have demonstrated its role in CRC tumorigenesis, as well as observing its overexpression in metastatic lymph nodes.

Research Area

Cancer Biology

Images & Validation

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Item 1 of 1

Tested Applications	ELISA
Dilution Range	ELISA: 2-4ug/ml (order BSA/azide-free format)
Reactivity	Human
Application Notes	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant TIMP1 antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Recombinant
Isotype	Mouse IgG1
Clone No.	rTIMP1/1710
Immunogen	Recombinant human protein was used as the immunogen for this recombinant TIMP1 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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SDS-PAGE analysis of purified, BSA-free recombinant TIMP1 antibody (clone rTIMP1/1710) as confirmation of integrity and purity.

Quick Database Links

UniProt

P01033

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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