Trovafloxacin mesylate

SKU: orb1308981

Description

Trovafloxacin is a broad-spectrum fluoroquinolone antibiotic that inhibits DNA gyrase and topoisomerase IV. It also specifically blocks the pannexin 1 (PANX1) channel with an IC50 of 4 µM. This dual activity makes it a useful research tool for studying bacterial replication and PANX1-mediated cellular signaling in vitro.

Research Area

Cell Biology, Infectious Disease & Virology, Molecular Biology, Pharmacology & Drug Discovery

Images & Validation

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Key Properties

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CAS Number	147059-75-4
MW	512.46
Purity	99.18% (May vary between batches)
Formula	C₂₁H₁₉F₃N₄O₆S
SMILES	CS(O)(=O)=O.[H][C@@]12CN(C[C@]1([H])[C@H]2N)c1nc2n(cc(C(O)=O)c(=O)c2cc1F)-c1ccc(F)cc1F
Target	Antibiotic,DNA/RNA Synthesis,TRP/TRPV Channel,Antibacterial,Topoisomerase
Solubility	DMSO:120 mg/mL (234.16 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:4 mg/mL (7.81 mM);H2O:18 mg/mL (35.12 mM)

Bioactivity

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Target IC50

PANX1:4 μM|pneumococcus:0.06-0.25 mg/mL (MIC)|Pneumococcal isolates:0.125 μg/mL (MIC)

In Vitro

Trovafloxacin mesylate is an inhibitor of TO-PRO-3 uptake by apoptotic cells and inhibits ATP release from apoptotic cells. Trovafloxacin mesylate does not inhibit caspase 3/7 activation, or caspase-mediated PANX1 cleavage during apoptosis. Trovafloxacin mesylate is equally active against both penicillin-susceptible and -resistant pneumococci, with MICs of 0.06-0.25 mg/mL reported for more than 700 isolates. The MICs of Trovafloxacin mesylate at which 90% of isolates are inhibited for 55 isolates of pneumococci is 0.125 μg/mL. Trovafloxacin mesylate prolongs TNF-induced activation of MAPKs and IKKα/β activation in HepG2 cells. Trovafloxacin mesylate (20 µM; 24 hours) and tumor necrosis factor (TNF; 4 ng/mL) incubation induces apoptosis and increases leakage of lactate dehydrogenase (LDH). Trovafloxacin mesylate (20 µM; 24 hours) and TNF (4 ng/mL) incubation increases expression of early NF-κB-related factors A20 and IκBα.

Storage & Handling

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Storage	keep away from direct sunlight \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Antibiotic, Bacterial, broad-spectrum, DNA, DNA Synthesis, DNASynthesis, DNA-gyrase, gyrase, Inhibitor, hepatotoxicity, NF-κB, LPS, PANX1, panx1, Pannexin 1 channel (PANX1), inhibit, pneumococci, TNF, Topo IV, Topoisomerase, topoisomerase-IV, pro-inflammatory, RNASynthesis, RNA Synthesis, TRPVChannel, TRPV Channel, TRPChannel, TRP Channel, Trovafloxacin, Trovafloxacin mesylate, Trovafloxacin Mesylate

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

Compound Identifiers

PubChem CID

62960 ↗

Key Properties

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No computed properties available.

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